Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Generation of Large Numbers of Myeloid…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection
Generation of Large Numbers of Myeloid Progenitors and Dendritic Cell Precursors from Murine Bone Marrow Using a Novel Cell Sorting Strategy
 

Generation of Large Numbers of Myeloid Progenitors and Dendritic Cell Precursors from Murine Bone Marrow Using a Novel Cell Sorting Strategy

Article DOI: 10.3791/57365-v • 09:06 min • August 10th, 2018
August 10th, 2018 •
1, 1
1Department of Biological Sciences, Auburn University

Chapters

Summary
Automatic Translation

Here we provide a method for identifying and isolating large numbers of GM-CSF driven myeloid cells using high speed cell sorting. Five distinct populations (Common myeloid progenitors, granulocyte/macrophage progenitors, monocytes, monocyte-derived macrophages, and monocyte-derived DCs) can be identified based on Ly6C and CD115 expression.

Tags

Keywords: Myeloid Progenitors Dendritic Cell Precursors Bone Marrow Cell Sorting Developmental Immunology Bone Marrow Transplantation Hind Legs Femur Tibia Marrow Cell Isolation Cell Culture Cell Preparation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter