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Assays til at studere rollen, som Vitronectin i bakteriel adhæsion og Serum modstand
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Chapters
- 00:04Title
- 00:49Detection of Vitronectin (Vn) Binding at the Bacterial Surface Using Flow Cytometry
- 02:18ELISA Analysis of the Interaction Between Recombinant Protein H (PH) and Vn
- 04:11Study of the Interaction Kinetics of Recombinant PH and Vn Using Bio-layer Interferometry
- 04:33Bacterial Adherence to the Vn-coated Glass Surfaces
- 05:32Vn-dependent Adherence of Bacteria to Epithelial Cells
- 07:29Analysis of Vn-dependent Resistance to the Bactericidal Activity of Human Serum
- 08:29Results: Interaction of Vitronectin and Bacteria
- 10:10Conclusion
Denne rapport beskriver protokoller for kendetegner interaktioner mellem bakteriel ydre Membranproteiner og menneskelige supplement regulator vitronectin. Protokollerne kan bruges til at studere den bindende reaktioner og biologiske funktion af vitronectin i enhver bakteriearter.
