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Visualizzazione Frequenza Stromule con microscopia a fluorescenza
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JoVE Journal Biology
Visualizing Stromule Frequency with Fluorescence Microscopy
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Visualizzazione Frequenza Stromule con microscopia a fluorescenza

DOI:
10.3791/54692-v

•

08:27 min

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November 23, 2016

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, ,
1Plant Gene Expression Center, Agricultural Research Service,USDA, 2Department of Plant and Microbial Biology,University of California, Berkeley

Chapters

  • 00:05Title
  • 00:53Preparing Leaf Samples for Visualization
  • 01:53Visualizing Stromules with Confocal Fluorescence Microscopy and Image Processing
  • 03:28Extracting Intact Chloroplasts to Visualize Stromule Dynamics
  • 05:58Results: Quantifying Stromule Frequency in N. benthamiana Leaves
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

Protocols to investigate the dynamics of chloroplast stromules, the stroma-filled tubules that extend from the surface of chloroplasts, are described.

Tags

ChloroplastStromuleFluorescence MicroscopyLive Cell ImagingNicotiana BenthamianaLeaf Sample PreparationChloroplast IsolationCytoskeletonZ-stackGFPConfocal Microscopy

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