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JoVE Journal Medicine
Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus
 

Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus

Article DOI: 10.3791/54718-v • 11:36 min • November 20th, 2016
November 20th, 2016 •
*1, *1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Stem Cell Institute, Katholieke Universiteit Leuven
* These authors contributed equally

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Summary
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Here we report a rapid and efficient gene editing method based on RMCE in the AAVS1 locus of human Pluripotent Stem Cells (hPSCs) that improves upon previously described systems. Using this technique, isogenic lines can be rapidly and reliably generated for proper comparative studies, facilitating transgenesis-mediated research with hPSCs.

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Keywords: Recombinant Human Pluripotent Stem Cells AAVS1 Locus Recombinase-mediated Cassette Exchange RMCE Transgenesis Stem Cell Biology Overexpression Knockdown Random Integration Karyotypes IMEFs ROCK Inhibitor Nucleofection Trypsin Accutase Feeder-free
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