A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:34Compound Microscope Setup
- 02:07Acquiring Images with a Compound Microscope
- 03:19Dissection Microscopy Setup
- 04:31Acquiring Images with a Dissection Microscope
- 08:51Results: Cell Counting Accuracy and Value of Calibration
- 10:12Conclusion
This paper describes the quantification of hemocytometer and migration/invasion micrographs through two new open-source ImageJ plugins Cell Concentration Calculator and migration assay Counter. Furthermore, it describes image acquisition and calibration protocols as well as discusses in detail the input requirements of the plugins.
