Journal
/
Biochemistry
/
An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity

An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity

DOI:
10.3791/54750-v

•

12:02 min

•

November 02, 2016

•

, ,
1Department of Chemistry,Durham University, 2School of Medicine, Pharmacy and Health,Durham University

Chapters

  • 00:05Title
  • 01:09Synthesis of a Linear Peptoid
  • 05:08Side-chain Deprotection and Cleavage from Resin
  • 06:32Cytotoxicity Assay against Leishmania mexicana Axenic Amastigotes
  • 09:12Results: Synthesis and Characterization of Peptoids and Their Cytotoxicity to Leishmania mexicana
  • 10:33Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol to synthesize peptoids with mixed cationic functionality in the same sequence is presented (lysine- and arginine-type monomers). Subsequent testing of these compounds against Leishmania mexicana, the protozoan parasites that cause cutaneous leishmaniasis, is also described.

Tags

Keywords: PeptoidsLysineArginineAnti-leishmanial ActivityCutaneous LeishmaniasisLeishmania MexicanaSubmonomer SynthesisFmoc-protected ResinPiperidineBromoacetic AcidDICAmine Solution

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/54750?article_type=v

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
check_url/54750?article_type=v

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
check_url/54750?article_type=v

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
check_url/54750?article_type=v

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
check_url/54750?article_type=v

Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants
check_url/54750?article_type=v

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
check_url/54750?article_type=v

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
check_url/54750?article_type=v

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
check_url/54750?article_type=v

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
check_url/54750?article_type=v

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

Read Article