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An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
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JoVE Journal Chemistry
An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

DOI:
10.3791/54759-v

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07:23 min

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October 10, 2016

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, , , , , ,
1Advanced Radiation Technology Institute,Korea Atomic Energy Research Institute, 2Department of Radiation Biotechnology and Applied Radioisotope Science,University of Science and Technology

Chapters

  • 00:05Title
  • 01:08Radiosynthesis of the 125I-labeled Azide Prosthetic Group
  • 03:59Radiolabeling of Dibenzocyclooctyne-group-modified Gold Nanoparticles via the Copper-free Click Reaction
  • 05:53Results: Validation of the Purified Radiolabeled Products
  • 06:32Conclusion

Summary

Automatic Translation

Automatic Translation

A detailed procedure for the synthesis of a 125I-labeled azide and the radiolabeling of dibenzocyclooctyne (DBCO)-group-conjugated, 13-nm-sized gold nanoparticles using a copper-free click reaction is described.

Tags

Keyword Extraction: RadiolabelingGold Nanoparticles125I-labeled AzideStrain Promoted Copper Free Click ReactionRadiochemistryRadio Isotope LabeledImaging ProbesPET/SPECTRadiochemical YieldRadiochemical PurityRadio Iodination ReactionChloramine TSodium MetabisulfiteHPLC AnalysisReversed Phase Analytical Radio HPLCPreparative Radio HPLCRadiochemical Yield

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