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Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications
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1Dept. of Clinical and Experimental Medicine,University of Pisa, 2OtoLab,Azienda Ospedaliero-Universitaria Pisana (AOUP), 3Dept. of Civil and Industrial Engineering,University of Pisa, 4Immunohematology Operative Unit,Azienda Ospedaliero-Universitaria Pisana (AOUP), 5Dept. Of Surgical, Medical, Molecular Pathology and Emergency Medicine,University of Pisa, 6II Orthopedic and Traumatologic Clinic,Azienda Ospedaliero-Universitaria Pisana (AOUP)
Chapters
- 00:05Title
- 00:57Preparation of Autologous Serum
- 02:20Isolation of hMSCs from Bone Marrow
- 03:42Culture of hMSCs in the Presence of Autologous Serum
- 05:19Osteogenic Pre-induction and Recovery of hMSCs
- 06:20Preparation of hMSCs/Fibrin Clot Constructs and Cell Viability Assay
- 07:30Results: hMSCs/Fibrin Clot Generation and Application
- 09:10Conclusion
Culturing human Mesenchymal Stromal Cells (hMSCs) with autologous serum, reduces the risks of rejection by xenogeneic material and other negative effects. It also allows for the recovery of a subset of mesodermal progenitors, which can deliver fresh hMSCs. Embedding hMSCs in an autologous fibrin clot enables easy handling and effective surgical implantation.
