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Mesenchymale stromal cellekultur og levering autolog Betingelser: En smart tilnærming for Ortopedisk Applications
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1Dept. of Clinical and Experimental Medicine,University of Pisa, 2OtoLab,Azienda Ospedaliero-Universitaria Pisana (AOUP), 3Dept. of Civil and Industrial Engineering,University of Pisa, 4Immunohematology Operative Unit,Azienda Ospedaliero-Universitaria Pisana (AOUP), 5Dept. Of Surgical, Medical, Molecular Pathology and Emergency Medicine,University of Pisa, 6II Orthopedic and Traumatologic Clinic,Azienda Ospedaliero-Universitaria Pisana (AOUP)
Chapters
- 00:05Title
- 00:57Preparation of Autologous Serum
- 02:20Isolation of hMSCs from Bone Marrow
- 03:42Culture of hMSCs in the Presence of Autologous Serum
- 05:19Osteogenic Pre-induction and Recovery of hMSCs
- 06:20Preparation of hMSCs/Fibrin Clot Constructs and Cell Viability Assay
- 07:30Results: hMSCs/Fibrin Clot Generation and Application
- 09:10Conclusion
Dyrking menneskelige Mesenchymale stromale celler (hMSCs) med autolog serum, reduserer risikoen for avvisning av xenogene materiale og andre negative effekter. Den gjør det også for utvinning av en undergruppe av mesodermal stamfedre, som kan levere ferske hMSCs. Embedding hMSCs i et autologt fibrinaggregat gjør håndteringen enkel og effektiv kirurgisk implantasjon.
