/

/

检查蛋白酶体大会与重组古菌蛋白酶体和非变性PAGE：该案例相结合的办法

JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biochemistry

Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach

Please note that all translations are automatically generated. Click here for the English version.

检查蛋白酶体大会与重组古菌蛋白酶体和非变性PAGE：该案例相结合的办法

DOI:

10.3791/54860-v

•

09:57 min

•

December 17, 2016

•

Dilrajkaur Panfair, Andrew R. Kusmierczyk

¹Department of Biology,Indiana University-Purdue University, Indianapolis (IUPUI)

Chapters

00:05Title
00:43Bacterial Lysis and Lysate Mixing
02:31Protein Purification via Immobilized Cobalt Affinity Resin (ICAR)
05:04Nondenaturing Polyacrylamide Gel Electrophoresis (PAGE)
08:09Results: A Combined Approach for Assaying Proteasome Assembly
09:24Conclusion

Summary

Automatic Translation

该协议使用两个亚基共表达和postlysis亚基重组蛋白酶组件的更彻底的检查混合。

Tags

Proteasome Assembly Recombinant Archaeal Proteasomes Nondenaturing PAGE Assembly Intermediates Eukaryotic Proteasome Archaeal 20S Proteasomes Coexpression Lysate Mixing Protein Complex Assembly

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

可逆二硫键交联胶束生产的简便和有效途径

使用脚手架脂质体重构脂质近端蛋白质 - 蛋白质相互作用<em>体外</em

多聚-PAGE：生物样品中的用于拍摄方法和解决蛋白复合体

用磷酸盐结合标记的 SDS-页 Rab10 磷酸化检测 LRRK2 活性

洗涤剂辅助重组重组果蝇阿拉他素到脂质混合测定脂质体

活联甘蔗，用于亲和色谱树脂的快速发展 - 抗体捕获作为案例研究

在氧化应激期间细胞粘附和纤维蛋白扩散动力学

一种可定制的酶化生产与纯化天然产物的方法

完全加工重组KRAS4b：分离和描述脱脂和甲基化蛋白

使用FlashPack方法进行简单的内部超高性能毛细管柱制造

Read Article