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一种优化的协议电泳迁移分析使用红外荧光染料标记的寡核苷酸

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

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一种优化的协议电泳迁移分析使用红外荧光染料标记的寡核苷酸

DOI:

10.3791/54863-v

•

09:58 min

•

November 29, 2016

•

Yi-Wen Hsieh, Amel Alqadah, Chiou-Fen Chuang

¹Department of Biological Sciences,University of Illinois at Chicago

Chapters

00:05Title
00:32Preparation of Gel, Infrared Fluorescent Dye-labeled Probes and Unlabeled Probes
04:49Binding Reaction and Electrophoresis
06:37Imaging
07:35Results: Electrophoretic Mobility Shift Assays Using Purified Proteins and Dye-labeled DNA Probes
08:57Conclusion

Summary

Automatic Translation

我们在这里描述使用纯化SOX-2蛋白与红外荧光染料标记的DNA探针作为案例研究解决的重要生物学问题一起荧光电泳迁移实验（FEMSA）的优化方案。

Tags

Electrophoretic Mobility Shift Assay Infrared Fluorescent Dye Oligonucleotides Protein-DNA Interactions Molecular Biology Biochemistry Non-radioactive Probes Native Polyacrylamide Gel TBE Buffer Glycerol Annealing Double-stranded DNA Probes Klenow Fragment EDTA PCR Unlabeled Probes

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