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Journal / Immunology and Infection / Optimization of a Quantitative…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Optimization of a Quantitative Micro-neutralization Assay
 

Optimization of a Quantitative Micro-neutralization Assay

Article DOI: 10.3791/54897-v • 10:09 min • December 14th, 2016
December 14th, 2016 •
1, 1, 1
1Mill Hill Laboratory, The Francis Crick Institute

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Summary
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This study describes an imaging-based micro-neutralization assay to analyze the antigenic relationships between viruses. The protocol employs a flatbed scanner and has four steps, including titration, titration quantitation, neutralization, and neutralization quantitation. The assay works well with current circulating influenza A(H1N1)pdm09, A(H3N2), and B viruses.

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Keywords: Quantitative Micro-neutralization Assay Influenza Viruses Antibody Characterization Antiviral Activity BSL 2 BSL 3+ Virus Growth Medium (VGM) Serial Dilutions Virus Infection Overlay PFA Fixation Permeabilization WHO Collaborating Center For Influenza
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