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在鼠耳皮肤的免疫反应的活体多光子成像诱导缺血再灌注损伤
JoVE Journal
Medicine
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JoVE Journal Medicine
Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses
DOI:

06:29 min

December 22, 2016

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Chapters

  • 00:05Title
  • 00:53Mouse Anesthesia and Depilation
  • 01:35Induction of Ischemia and Reperfusion Injury
  • 02:31Placement of the Ear on the Imaging Platform
  • 04:33Results: Neutrophil Recruitment in Ischemia-reperfusion Injury
  • 05:20Conclusion

Summary

Automatic Translation

这个协议描述了在使用磁铁的夹紧小鼠耳部皮肤缺血再灌注(IR)模型的诱导。使用定制的活体成像模型,我们研究的体内炎症反应再灌注后。这项技术的发展背后的基本原理是延长白细胞如何皮肤缺血再灌注损伤反应的认识。

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