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Genetics
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使用周囲粒子状物質によって誘発される染色体異常の解析<em>インビトロ</em>システム
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Genetics
Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an
In Vitro
System
Please note that all translations are automatically generated.
Click here for the English version.
使用周囲粒子状物質によって誘発される染色体異常の解析<em>インビトロ</em>システム
DOI:
10.3791/54969-v
•
08:48 min
•
December 21, 2016
•
Isabelle R. Miousse
,
Igor Koturbash
,
Marie-Cécile Chalbot
,
Martin Hauer-Jensen
,
Ilias Kavouras
,
Rupak Pathak
1
Department of Occupational and Environmental Health
,
University of Arkansas for Medical Sciences
,
2
Department of Environmental Health Sciences
,
University of Alabama at Birmingham
,
3
Division of Radiation Health
,
University of Arkansas for Medical Sciences
Chapters
00:05
Title
00:35
Particle Exposure
02:12
Cell Treatment with Particulate Matter
03:21
Cell Harvest, Hypotonic Treatment, and Fixation
05:35
Slide Cleaning and Metaphase Spread Preparation
07:12
Results: Chromosomal Aberrations from Particulate Matter on Metaphase Spreads
08:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは、周囲の空気の粒子状物質で処理した後、RAW264.7マウスマクロファージにおける
in vitroで
染色体異常の定量および特徴付けのための手法について説明します。
Tags
Chromosomal Aberrations
Particulate Matter
In Vitro
Air Pollution
Cell Culture
Genotoxicity
Metaphase Arrest
Trypsin
Cell Suspension
Cell Density
Cell Incubation
PBS
Colcemid
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check_url/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations
in vitro
転写アッセイおよび創薬への応用
check_url/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations
複数の蛍光によるインター染色体安定型異常の検出<em>その場で</em>ハイブリダイゼーション(mFISH)と照射されたマウスにおけるスペクトル核型分析(SKY)
check_url/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations
クロマチン免疫沈降法を用いたc-KITリガンドプロモーターの解析
check_url/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations
新規トランスポゾンを介したアプローチを使用して
エシェリヒア属大腸菌
DNA 要素の最適な染色体の場所の決定
check_url/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations
In Vivo
モデル システムとしてゼブラフィッシュを用いた DNA 複製タイミングのプロファイリング
check_url/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations
クロマチンのループ構造の再編を CRISPR 媒介
check_url/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations
回転運動の定量化システム (リクエスト) を用いた
キイロショウジョウバエ
の運動レベルの測定
check_url/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations
MRNA の構造変化を誘導培養と細胞の形で使用して小分子を調査するには
check_url/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations
腫瘍性細胞行動の尺度としてのインビトロ侵血アッセイにおけるマウス乳腺腫瘍細胞の使用
check_url/54969?article_type=v&slug=analysis-ambient-particulate-matter-induced-chromosomal-aberrations
ビオチン標識を用いてタンパク質核酸相互作用を研究するインビトロ生化学アッセイ
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