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触发细胞应激和死亡使用传统的紫外线激光共聚焦显微镜
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy
DOI:

10:18 min

February 03, 2017

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Chapters

  • 00:05Title
  • 01:21Prepare Zebrafish for Mounting and UV Cell Ablation
  • 04:18Confocal Microscope Set Up and Targeted Laser Ablation
  • 07:25Results: UV Laser Microscopy Induced Cell Stress and Death
  • 09:03Conclusion

Summary

Automatic Translation

Targeted manipulations to cause directed stress or death in individual cells have been relatively difficult to accomplish. Here, a single-cell-resolution ablation approach to selectively stress and kill individual cells in cell culture and living animals is described based on a standard confocal UV laser.

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