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Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice
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Chapters
- 00:00Title
- 00:09Introduction
- 01:03Cannulation and Perfusion
- 03:55Purification and Handpicking
- 06:30Troubleshooting: Common Bile Duct Distended During Perfusion
- 07:03Troubleshooting: Duodenum Distended During Perfusion
- 07:37Islet Transplantation at EFP Site
- 10:23Results
- 11:29Conclusion
This protocol demonstrates murine islet isolation and seeding onto a decellularized scaffold. Scaffold-supported islets were transplanted into the epididymal fat pad of streptozotocin (STZ)-induced diabetic mice. Islets survived at the transplantation site and reversed the hyperglycemic condition.
