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Testa Vascular Invasive cancercellers förmåga i zebrafisk (<em> Danio rerio</em>)
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Testing the Vascular Invasive Ability of Cancer Cells in Zebrafish (Danio Rerio)
DOI:

07:31 min

November 03, 2016

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Chapters

  • 00:05Title
  • 00:40Injecting Cancer Cells into the Pre-cardiac Sinus of Zebrafish Embryos
  • 03:58Scoring Extravasation
  • 04:37Mounting Embryos onto Slides and Subsequent Fluorescence Imaging
  • 05:34Results: Vascular Invasion of Cancer Cell Lines in Zebrafish Embryos
  • 06:50Conclusion

Summary

Automatic Translation

Denna metod använder zebrafisk embryon för att effektivt testa den vaskulära invasiva förmågan hos cancerceller. Fluorescerande cancerceller injiceras i prekardiellt sinus eller gulesäcken utvecklings embryon. Cancercell vaskulär invasion och extravasering bedöms via fluorescensmikroskopi av svansregionen 24-96 timmar senare.

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