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生产，结晶和结构测定<em>℃。难辨</em>通过Microseeding和锌SAD PPEP-1
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Biochemistry
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JoVE Journal Biochemistry
Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
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生产，结晶和结构测定<em>℃。难辨</em>通过Microseeding和锌SAD PPEP-1

DOI:
10.3791/55022-v

•

13:34 min

•

December 30, 2016

•

, , ,
1Institute of Biochemistry,University of Cologne

Chapters

  • 00:05Title
  • 01:23Initial Screening of rPPEP-1 and Co-crystallization of rPPEP-1 with Ligands Using Commercial Screens
  • 03:54Crystal Optimization Using Microseeding
  • 07:35Crystal Mounting
  • 10:45Results: Evaluation of rPPEP-1 Crystal Growth Using Optimization Procedure
  • 12:48Conclusion

Summary

Automatic Translation

Automatic Translation

Proline-proline endopeptidase-1 (PPEP-1) is a secreted metalloprotease and promising drug-target from the human pathogen Clostridium difficile. Here we describe all methods necessary for the production and structure determination of this protein.

Tags

CrystallizationStructure DeterminationC. Difficile PPEP-1MicroseedingZinc-SADProtein PurificationProtein ConcentrationSitting DropCrystallization ScreeningCrystal GrowthProtein Structure

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