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映射的适体对ATP的结合部位使用MicroScale程序热泳
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Biochemistry
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JoVE Journal Biochemistry
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
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映射的适体对ATP的结合部位使用MicroScale程序热泳

DOI:
10.3791/55070-v

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08:09 min

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January 07, 2017

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,
12bind GmbH

Chapters

  • 00:05Title
  • 00:46MicroScale Thermophoresis (MST) Preparation
  • 03:46EC50 Determination by MST Measurements
  • 06:33Results: Characterization of the Binding Site of the DH25.42 DNA Aptamer on ATP
  • 07:58Conclusion

Summary

Automatic Translation

Automatic Translation

MicroScale Thermophoresis (MST) is a sensitive technology to characterize aptamer-target interactions. This manuscript describes an MST protocol to characterize aptamer-small molecule interactions.

Tags

Keywords: AptamerATPMicroscale ThermophoresisBinding Site MappingCy5 Labeled DNA AptamerLigand Serial DilutionFluorescence SignalCapillary ScanMolecular Interactions

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