Journal
/
Immunology and Infection
/
Murine Full-thickness Skin Transplantation
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Murine Full-thickness Skin Transplantation

Murine Full-thickness Skin Transplantation

DOI:
10.3791/55105-v

•

07:59 min

•

January 02, 2017

•

, , , , , ,
1Sidney-Kimmel Comprehensive Cancer Center,Johns Hopkins University School of Medicine, 2Department of Liver and Transplantation Surgery, Chang-Gung Transplantation Institute, Chang-Gung Memorial Hospital,Chang-Gung University College of Medicine, 3Vascularized Composite Allotransplantation Laboratory, Department of Plastic and Reconstructive Surgery,Johns Hopkins University School of Medicine

Chapters

  • 00:05Title
  • 01:03Donor Skin Harvest
  • 02:06Recipient Skin Transplant
  • 04:00Postoperative Care
  • 05:09Results: Full-thickness Skin Grafts
  • 07:00Conclusion

Summary

Automatic Translation

Automatic Translation

Murine full-thickness skin transplantation is a well-established model to study rejection in an alloimmune setting. Here, we provide a tutorial of each step involved in performing a BALB/c-->C57BL/6 full-thickness skin transplant.

Tags

MurineFull-thickness Skin TransplantationAlloimmune ResponseGraft RejectionDonor-matched Skin GraftThird-party Skin GraftPanniculus CarnosusGraft BedRevascularization

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55105?article_type=v

Murine Model of CD40-activation of B cells
check_url/55105?article_type=v

Granulocyte-dependent Autoantibody-induced Skin Blistering
check_url/55105?article_type=v

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice
check_url/55105?article_type=v

Isolation and Transplantation of Different Aged Murine Thymic Grafts.
check_url/55105?article_type=v

A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin
check_url/55105?article_type=v

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
check_url/55105?article_type=v

Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses
check_url/55105?article_type=v

Renal Subcapsular Transplantation of 2′-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice
check_url/55105?article_type=v

Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E
check_url/55105?article_type=v

Optimization of the Cuff Technique for Murine Heart Transplantation

Read Article