Journal
/
Biochemistry
/
实验室规模的生产和治疗性抗体的纯化
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Laboratory Scale Production and Purification of a Therapeutic Antibody
Please note that all translations are automatically generated. Click here for the English version.

实验室规模的生产和治疗性抗体的纯化

DOI:
10.3791/55153-v

•

09:54 min

•

January 24, 2017

•

, , , ,
1Faculty of Pharmacy,University of Sydney

Chapters

  • 00:05Title
  • 00:38Stable Transfection of HEK-293 Cells
  • 03:59Large Scale Antibody Production from Batch Overgrowth Culture
  • 05:12Antibody Purification by Affinity Chromatograpy Using an Automated Fast Protein Liquid Chromatography System
  • 07:02Results: Laboratory-scale Production and Purification of Antibody
  • 08:50Conclusion

Summary

Automatic Translation

Automatic Translation

这个协议描述在哺乳动物表达系统中生产的治疗性抗体的。所描述的方法包括制备载体DNA，稳定转染和人胚肾293细胞系的无血清适应的，设置大规模培养和纯化使用亲和层析组成。

Tags

Antibody ProductionTherapeutic AntibodySerum-free ExpressionHEK-293 CellsStable Cell LineTransfectionPolyethylenimine (PEI)Hygromycin BSerum-free AdaptationPurification

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

从外膜囊泡内蛋白质导演包装<em>大肠杆菌</em>：设计，生产和纯化

细菌内膜显示筛查抗体片段的图书馆

生产，结晶和结构测定<em>℃。难辨</em>通过Microseeding和锌SAD PPEP-1

S100A12的表达，纯化和抗菌活性

化学感受器配体结合域的高效复用与纯化方法

光谱分析 TRPV1 的纯化与重构

烟草 Benthamiana叶在制备鳞片上的瞬态表达

利用病毒衍生系统在大肠杆菌中大规模生产圆形脚手架上的重组 rna

无核酸酶的表达和纯化原氧基质 3，4-二氧酶

一种可定制的酶化生产与纯化天然产物的方法

Read Article