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JoVE Journal Developmental Biology
Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
 

Effective Isolation of Functional Islets from Neonatal Mouse Pancreas

Article DOI: 10.3791/55160-v • 07:04 min • January 6th, 2017
January 6th, 2017 •
1, 1
1Department of Cell and Development Biology, Vanderbilt University Medical School

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Summary
Automatic Translation

We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.

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Keywords: Neonatal Mouse Pancreas Islet Isolation Collagenase Digestion HBSS RPMI 1640 Polysucrose Sodium Diatrazoate Insulin Secretion Gene Expression Paracell Survival Functional Maintenance
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