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Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
DOI:

12:11 min

May 11, 2017

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Chapters

  • 00:05Title
  • 01:01Ultrasound-guided Ovum Pick-up (OPU)
  • 05:06In Vitro Maturation
  • 06:18Immunofluorescent Staining of Oocytes
  • 09:57Results: Chromosome Segregation, Spindle Length, and Acetylation of Oocytes Matured In Vitro and In Vivo
  • 11:04Conclusion

Summary

Automatic Translation

This manuscript describes an experimental approach to morphologically and biochemically characterize horse oocytes. Specifically, the present work illustrates how to collect immature and mature horse oocytes by ultrasound-guided ovum pick-up (OPU) and how to investigate chromosome segregation, spindle morphology, global histone acetylation, and mRNA expression.

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