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马卵母细胞染色体分离,组蛋白乙酰化和主轴形态分析
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes
DOI:

12:11 min

May 11, 2017

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Chapters

  • 00:05Title
  • 01:01Ultrasound-guided Ovum Pick-up (OPU)
  • 05:06In Vitro Maturation
  • 06:18Immunofluorescent Staining of Oocytes
  • 09:57Results: Chromosome Segregation, Spindle Length, and Acetylation of Oocytes Matured In Vitro and In Vivo
  • 11:04Conclusion

Summary

Automatic Translation

该手稿描述了一种用于形态和生物化学表征马卵母细胞的实验方法。具体来说,本文通过超声引导的卵子吸收(OPU)如何收集不成熟和成熟的马卵母细胞,以及如何调查染色体分离,纺锤形态,全局组蛋白乙酰化和mRNA表达。

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