Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
 

Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination

Article DOI: 10.3791/55284-v 08:52 min February 18th, 2017
February 18th, 2017

Chapters

Summary

Liver injuries are accompanied by progenitor cell expansion that represents a heterogeneous cell population. Novel classification of this cellular compartment allows for the distinguishing of multiple subsets. The method described here illustrates the flow cytometry analysis and high purity isolation of various subsets that can be used for further assays.

Tags

Keywords: Liver Progenitor Cells Liver Digestion Protocol Flow Cytometry Surface Marker Combination Cell Isolation Cell Biology Hematopoietic Cells Endothelial Cells Mouse Liver Tissue Dissociation Cell Suspension Cell Counting Propidium Iodide
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter