Journal
/
Developmental Biology
/
Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning

Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning

DOI:
10.3791/53764-v

•

11:11 min

•

February 17, 2016

•

, , ,
1Neurology,Johns Hopkins University, School of Medicine

Chapters

  • 00:05Title
  • 00:50Rat Brain Dissection
  • 04:20Enzymatic and Mechanical Tissue Dissociation
  • 05:26Anti-A2B5 Bead Application, Column Purification, and Plating
  • 06:29Induction of OPC Differentiation and Fixation with 4% Paraformaldehyde
  • 07:38Immunocytochemistry and Qualification of Myelin Basic Protein
  • 09:25Results: OPC Culture and Differentiation Procedure
  • 10:29Conclusion

Summary

Automatic Translation

Automatic Translation

Oligodendrocytes are the myelinating cells of the central nervous system. This protocol describes a method for the isolation and culture of their precursors, oligodendrocyte progenitor cells, from rat cortices, as well as a fast and reliable quantitative method to evaluate oligodendrogenesis in vitro in response to experimental factors.

Tags

Oligodendrocyte Progenitor CellsOligodendrogenesisSpinal Cord InjuryNeonatal HypoxiaDemyelinating DiseasesTransverse MyelitisMultiple SclerosisRat PupsDissectionPBSPetri DishCraniumCerebellumOlfactory BulbsHypothalamusMidbrainHippocampus

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/53764?article_type=v

Organotypic Slice Cultures for Studies of Postnatal Neurogenesis
check_url/53764?article_type=v

Delivery of In Vivo Acute Intermittent Hypoxia in Neonatal Rodents to Prime Subventricular Zone-derived Neural Progenitor Cell Cultures
check_url/53764?article_type=v

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord
check_url/53764?article_type=v

Generation of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis
check_url/53764?article_type=v

Using Primary Neurosphere Cultures to Study Primary Cilia
check_url/53764?article_type=v

Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture

Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs
check_url/53764?article_type=v

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
check_url/53764?article_type=v

Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells
check_url/53764?article_type=v

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Read Article