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Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs
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Developmental Biology
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JoVE Journal Developmental Biology
Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs

Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs

DOI:
10.3791/57527-v

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08:17 min

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June 01, 2018

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, ,
1Department of Stem Cell Biology and Regenerative Medicine,Keck School of Medicine of the University of Southern California, 2Caruso Department of Otolaryngology-Head and Neck Surgery,Keck School of Medicine of the University of Southern California, 3Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience,The Rockefeller University

Chapters

  • 00:04TItle
  • 00:58Dissection of Vestibular and Auditory Organs
  • 03:09Placing the Vestibular or the Auditory Sensory Organ in a Collagen I Gel
  • 04:45Viral Injections in Three-dimensional Cultures of Vestibular and Auditory Sensory Organs
  • 06:06Results: Use of Three-dimensional Organotypic Culture in Collagen I Gel to Study the Architecture and Viral Infections of the Vestibular and Cochlear Sensory Organs
  • 07:40Conclusion

Summary

Automatic Translation

Automatic Translation

Three-dimensional organotypic cultures of the murine utricle and cochlea in optically clear collagen I gels preserve innate tissue morphology, allow for mechanical stimulation through adjustment of matrix stiffness, and permit virus-mediated gene delivery.

Tags

3D Organotypic CultureVestibular OrganAuditory OrganInner EarCochleaUtricleSensory Organ DevelopmentMechanical ForceIn Vitro Model

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