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Bioengineering

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JoVE Journal Bioengineering
Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
 

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Article DOI: 10.3791/55287-v • 11:33 min • March 9th, 2017
March 9th, 2017 •
1, 1, 1
1Department of Chemistry, Tufts University

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Summary
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We detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our approach to device assembly is a type of multilayer, additive manufacturing. We demonstrate a sandwich immunoassay to provide representative results for these types of paper-based devices.

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Keywords: Paper-based Microfluidics Immunoassays Point-of-care Fabrication Three-dimensional Devices Parallel Manufacturing Qualitative Filter Paper Wax Printing Nylon Membrane Capture Layer Gravity Convection Oven
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