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JoVE Journal
Immunology and Infection

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Identification of Plasmodesmal Localization Sequences in Proteins In Planta
 

Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Article DOI: 10.3791/55301-v 08:07 min August 15th, 2017
August 15th, 2017

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Summary

Plant intercellular connections, the plasmodesmata (Pd), play central roles in plant physiology and plant-virus interactions. Critical to Pd transport are sorting signals that direct proteins to Pd. However, our knowledge about these sequences is still in its infancy. We describe a strategy to identify Pd localization signals in Pd-targeted proteins.

Tags

Keywords: Plasmodesmata Localization Signal Intercellular Transport Protein Targeting Nicotiana Benthamiana Fluorescent Tagging Agrobacterium Tumefaciens Transient Expression
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