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JoVE Journal
Developmental Biology

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Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry
 

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry

Article DOI: 10.3791/55305-v 08:59 min July 17th, 2017
July 17th, 2017

Chapters

Summary

While infiltrating macrophages are continuously recruited to adult tissues from circulating precursors, resident macrophages seed their tissue during development, where they are maintained without further input from progenitors. The progenitors for resident macrophages were recently identified. Here, we present methods for the genetic fate mapping of the resident macrophage progenitors.

Tags

Keywords: Erythromyeloid Progenitors Fate Mapping Flow Cytometry Hematopoietic Progenitors Hematopoietic Stem Cells Immunology Mouse Embryo Yolk Sac Resident Macrophages Dissection Fetal Liver EDTA Tissue Digestion
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