Journal
/
Biology
/
Forberedelse af meiotiske kromosom spreder sig fra musen Spermatocytes
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes

Forberedelse af meiotiske kromosom spreder sig fra musen Spermatocytes

DOI:
10.3791/55378-v

•

06:38 min

•

November 22, 2017

•

, , , ,
1Department of Biochemistry & Molecular Biology,Drexel University College of Medicine, 2Department of Obstetrics & Gynecology,Drexel University College of Medicine

Chapters

  • 00:05Title
  • 00:52Spermatocyte Nuclei Spreading
  • 04:44Results: Representative Wild-type, Juvenile Mouse, Surface-Spread Nuclei
  • 05:32Conclusion

Summary

Automatic Translation

Automatic Translation

Meiose er den udviklingsmæssige proces hvorved mælke dannes ved en enkelt runde af DNA-replikation og to efterfølgende runder af kromosom adskillelse. Pattedyr meiose kan undersøges ved at benytte en teknik til at forberede meiotiske kromosom spreads. Her viser vi en metode til at forberede overflade-spredning kerner fra mus spermatocytes.

Tags

Keywords: Meiotic Chromosome SpreadsMouse SpermatocytesReproductive BiologyMeiotic RecombinationChromosome SegregationSeminiferous TubulesHypotonic Extraction BufferFixative SolutionTeflon Printed Slides

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55378?article_type=v

Preparation of Dissociated Mouse Cortical Neuron Cultures
check_url/55378?article_type=v

Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
check_url/55378?article_type=v

Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
check_url/55378?article_type=v

Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling
check_url/55378?article_type=v

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
check_url/55378?article_type=v

Flow Cytometry Purification of Mouse Meiotic Cells
check_url/55378?article_type=v

Generering af mus afledt fra induceret pluripotente stamceller
check_url/55378?article_type=v

Kromosom Forberedelse Fra dyrkede celler
check_url/55378?article_type=v

En hurtigt Air-tør droppe kromosom fremstillingsmetode Egnet til FISH i planter
check_url/55378?article_type=v

Meiotiske Spindel Vurdering i museoocytter af siRNA-medieret Silencing

Read Article