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Journal
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Biochemistry
/
增强组织样品使用组合前体同位素标记和标记等压的复用(cPILOT)
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
Please note that all translations are automatically generated.
Click here for the English version.
增强组织样品使用组合前体同位素标记和标记等压的复用(cPILOT)
DOI:
10.3791/55406-v
•
09:06 min
•
May 01, 2017
•
Christina D. King
,
Joseph D. Dudenhoeffer
,
Liqing Gu
,
Adam R. Evans
,
Renã A. S. Robinson
1
Department of Chemistry
,
University of Pittsburgh
,
2
SGS North America Inc.
,
3
Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science
,
Janssen Research and Development
Chapters
00:05
Title
01:14
Protein Extraction and Generation of Peptides for Chemical-tagging
02:05
Sample Digestion
03:16
Dimethylation Labeling (N-termini)
05:13
Isobaric Tagging (Lys residues)
06:12
Liquid Chromatography – Tandem Mass Spectrometry (LC-MS/MS) and MS
3
07:02
Results: cPILOT Can Be Used to Analyze Tissues from an Alzheimer’s Disease Mouse Model and Wild-type Controls
08:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
合并前体同位素标记和等压标记(cPILOT)是定量蛋白质组学战略,增强同量异位标记的样品复用能力。这个协议描述cPILOT的对组织从阿尔茨海默氏病小鼠模型中和野生型对照的应用。
Tags
Keywords: Enhanced Sample Multiplexing
Combined Precursor Isotopic Labeling
Isobaric Tagging
CPILOT
Alzheimer’s Disease
Mouse Model
Protein Changes
Aging
Neurodegenerative Disease
Cancer
Pathogenesis
Diagnosis
Prognosis
Therapeutic Targets
Acetylation
Protein Nitration
Brain
Heart
Liver
BCA Assay
Protein Concentration
Dithiothreitol
Iodoacetamide
L-cysteine
Trypsin Digestion
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