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病毒衍生肽修饰抗体结合物对增强细胞积累和改善肿瘤靶向性的初步评价
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Bioengineering
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JoVE Journal Bioengineering
Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting
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病毒衍生肽修饰抗体结合物对增强细胞积累和改善肿瘤靶向性的初步评价

DOI:
10.3791/55440-v

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11:58 min

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March 08, 2018

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, , , , , ,
1Department of Nuclear Medicine and Radiobiology,Université de Sherbrooke, 2Sherbrooke Molecular Imaging Center (CIMS),Université de Sherbrooke, 3Sherbrooke Institute of Pharmacology

Chapters

  • 00:05Title
  • 01:07Confocal Microscopy for Intracellular Accumulation Evaluation
  • 04:49Radiolabeled AC Construction and Cellular Fractionation for the Evaluation of 64Cu Intracellular Delivery Efficiency
  • 08:23PET Imaging Evaluation of Tumor Targeting
  • 09:22Results: Evaluation of Intracellular Accumulation of Antibody Conjugates
  • 11:30Conclusion

Summary

Automatic Translation

Automatic Translation

病毒衍生肽与抗体结合 (ACs) 是一种获得动力的方法, 由于可能提供的分子负载增加肿瘤细胞积累。利用常用的方法来评估肽共轭、AC 和有效载荷的胞内积累和肿瘤靶向, 这项协议帮助研究人员在关键的初步发展阶段。

Tags

Antibody-conjugatesViral-derived PeptidesCellular AccumulationTumor TargetingPET ImagingNuclear LocalizationTF1A CellsColic-acidMonoclonal AntibodiesTrypsinEDTARPMI1640PFASucroseTriton X-100Alexa Fluor 647

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