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面对
小鼠血液制备
JoVE Journal
Biology
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JoVE Journal
Biology
En Face
Preparation of Mouse Blood Vessels
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面对
小鼠血液制备
DOI:
10.3791/55460-v
•
10:01 min
•
May 19, 2017
•
Kyung Ae Ko
,
Keigi Fujiwara
,
Sunil Krishnan
,
Jun-ichi Abe
1
Department of Cardiology, Division of Internal Medicine
,
University of Texas MD Anderson Cancer Center
,
2
Department of Radiation Oncology
,
University of Texas MD Anderson Cancer Center
Chapters
00:05
Title
00:53
Left Partial Carotid Artery Ligation
03:29
En Face
Immunostaining
08:09
Results: Representative
En Face
Immunofluorescent Imaging
09:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
描述了制备小鼠颈动脉和主动脉的方法。当用特异性抗体进行免疫荧光染色时,这样的制剂使得我们能够研究蛋白质的定位和通过共聚焦显微镜在整个血管壁内鉴定细胞类型。
Tags
En Face
Mouse Blood Vessels
Aorta
Carotid Artery
Immunostaining
Vascular Cells
Carotid Artery Ligation
Surgical Procedure
Anesthetized Mouse
Salivary Glands
Internal Carotid Artery
External Carotid Artery
Ligation
Occipital Artery
Sutures
Thoracic Cavity
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