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面对小鼠血液制备
JoVE Journal
Biology
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JoVE Journal Biology
En Face Preparation of Mouse Blood Vessels
DOI:

10:01 min

May 19, 2017

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Chapters

  • 00:05Title
  • 00:53Left Partial Carotid Artery Ligation
  • 03:29En Face Immunostaining
  • 08:09Results: Representative En Face Immunofluorescent Imaging
  • 09:18Conclusion

Summary

Automatic Translation

描述了制备小鼠颈动脉和主动脉的方法。当用特异性抗体进行免疫荧光染色时,这样的制剂使得我们能够研究蛋白质的定位和通过共聚焦显微镜在整个血管壁内鉴定细胞类型。

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