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使用全身免疫组织化学方法研究胆道的神经支配<em> Suncus murinus</em

JoVE Journal
Biology

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JoVE Journal Biology

Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus

Please note that all translations are automatically generated. Click here for the English version.

使用全身免疫组织化学方法研究胆道的神经支配<em> Suncus murinus</em

DOI:

10.3791/55483-v

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07:23 min

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June 15, 2017

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Ke Ren, Yidan Dai, Kai Yi, Masanobu Kinoshita, Masahiro Itoh, Ichiro Sakata, Takafumi Sakai, Shuang-Qin Yi

¹Department of Frontier Health Sciences, Graduate School of Human Health Sciences,Tokyo Metropolitan University, ²Department of Anatomy,Tokyo Medical University, ³Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering,Saitama University

Chapters

00:05Title
00:54Whole-mount Immunohistochemistry
05:12Results: Representative Neurofilament+ Nerve Fiber Extrahepatic Bilary Tract Staining in S. murinus
06:33Conclusion

Summary

Automatic Translation

一种全身免疫组织化学方法，可以显示Suncus murinus肝外胆道中的神经丝蛋白表达。在这里介绍。该方案可用于分析小鼠或其他物种中所有内脏器官的神经支配。

Tags

Keywords: Whole-mount Immunohistochemistry Biliary Tract Suncus Murinus Nerve Distribution Visceral Organs Neoprene Latex Gallbladder Orthoperiodic Acid Papillon Paraformaldehyde Sucrose Incubations

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