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Brug af en helmonteret immunhistokemisk metode til at studere indervation af galdevejen i<em> Suncus murinus</em
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JoVE Journal Biology
Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus

Brug af en helmonteret immunhistokemisk metode til at studere indervation af galdevejen i<em> Suncus murinus</em

DOI:
10.3791/55483-v

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07:23 min

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June 15, 2017

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, , , , , , ,
1Department of Frontier Health Sciences, Graduate School of Human Health Sciences,Tokyo Metropolitan University, 2Department of Anatomy,Tokyo Medical University, 3Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering,Saitama University

Chapters

  • 00:05Title
  • 00:54Whole-mount Immunohistochemistry
  • 05:12Results: Representative Neurofilament+ Nerve Fiber Extrahepatic Bilary Tract Staining in S. murinus
  • 06:33Conclusion

Summary

Automatic Translation

Automatic Translation

En helmonteret immunohistokemisk tilgang til at visualisere neurofilamentproteinekspression i det ekstrahepatiske galde i Suncus murinus. Er præsenteret her. Denne protokol kan bruges til at analysere innerveringen af ​​alle viscerale organer i S. murinus eller andre arter.

Tags

Keywords: Whole-mount ImmunohistochemistryBiliary TractSuncus MurinusNerve DistributionVisceral OrgansNeoprene LatexGallbladderOrthoperiodic AcidPapillonParaformaldehydeSucrose Incubations

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