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La espectrometría de masa y enfoques basados en luminogénico para caracterizar la fase I metabólico de Competencia<em> In Vitro</em> Cultivos Celulares
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Chapters
- 00:05Title
- 00:48Cell Culture
- 02:49CYP Activity Quantification in Cell Cultures: Metabolic Probes, Inducers, and Inhibitors
- 04:30CYP Activity Quantification in Cell Cultures: Medium Treatment
- 05:40CYP Activity Quantification in Cell Cultures: Mass Spectrometry (MS)-based Quantification
- 06:49CYP Activity Quantification in Cell Cultures: Probes Using Luminescence-based Methods
- 08:10Results: Quantification of Metabolites Produced by tha Activity of CYP Enzymes Following Cell Incubation with CYP Inducers, Substrates, and Inhibitors
- 09:35Conclusion
Competencia metabólica de los sistemas in vitro es un requisito clave para la biotransformación y la disposición de fármacos y sustancias tóxicas. En este protocolo, se describe la aplicación de sondas metabólicos de referencia para evaluar la fase I metabolismo en cultivos celulares.
