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S100A12的表达，纯化和抗菌活性
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Biochemistry
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JoVE Journal Biochemistry
Expression, Purification, and Antimicrobial Activity of S100A12
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S100A12的表达，纯化和抗菌活性

DOI:
10.3791/55557-v

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11:10 min

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May 13, 2017

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, , , ,
1Department of Life and Physical Sciences,Fisk University, 2Tennessee Valley Healthcare Systems,U. S. Dept. of Veterans Affairs, 3Department of Medicine – Division of Infectious Diseases,Vanderbilt University Medical School, 4Department of Biochemistry,Vanderbilt University

Chapters

  • 00:05Title
  • 01:17Expression of S100A12
  • 03:20Purification of S100A12 Using Low Pressure Chromatography
  • 06:27Antimicrobial Activity Assays
  • 07:58Results: The Expression, Purification, and Antimicrobial Activity of S100A12
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们提出了一种表达和纯化S100A12（钙粒蛋白C）的方法。我们描述了一种测定其对人类幽门螺杆菌的抗微生物活性的方案 。

Tags

Keywords: S100A12Antimicrobial PeptideProtein ExpressionProtein PurificationAntimicrobial ActivityMetal SequestrationOligomerizationCalprotectinBL21 DE3 CellsAutoinduction MediaCentrifugationSonicationAffinity ChromatographySize Exclusion Chromatography

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