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A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
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Developmental Biology
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JoVE Journal Developmental Biology
A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

DOI:
10.3791/55565-v

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10:07 min

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May 17, 2017

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, , , , , ,
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics,Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry,The Chinese University of Hong Kong, 4School of Biomedical Engineering,Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center,Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College

Chapters

  • 00:05Title
  • 01:52Human Articular Cartilage Tissue Collection and Chondrocyte Culture
  • 03:33Genomic DNA Extraction
  • 05:47Dot Blot nalysis
  • 07:55Results: DNA Methylation Increases During Chondrocyte Dedifferentiation
  • 08:45Conclusion

Summary

Automatic Translation

Automatic Translation

We present a method to quantify DNA methylation based on the 5-methylcytosine (5-mC) dot blot. We determined the 5-mC levels during chondrocyte dedifferentiation. This simple technique could be used to quickly determine the chondrocyte phenotype in ACI treatment.

Tags

5-mC Dot BlotDNA MethylationChondrocyte DedifferentiationAutologous Chondrocyte ImplantationCartilage DefectsOsteoarthritisGenomic DNANylon MembraneCollagenase IICell CultureCell Passage

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