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Journal / Developmental Biology / In Vitro Growth of Mouse Preantral Follicles…
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Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
 

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity

Article DOI: 10.3791/55641-v • 10:48 min • December 17th, 2017
December 17th, 2017 •
1, 1, 2, 1, 3, 4, 5, 6, 1,7
1Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University, 2Department of Orthopaedics, The Second Affiliated Hospital of Wenzhou Medical University, 3Department of Obstetrics, The First Affiliated Hospital of Wenzhou Medical University, 4School of Laboratory Medicine and Life Science, Wenzhou Medical University, 5School of Pharmaceutical Science, Wenzhou Medical University, 6Stem Cells and Genetic Engineering Group, AgriBioscience Research Centre, Department of Economic Development, Jobs, Transport and Resources, 7Department of Histology and Embryology, Wenzhou Medical University

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Summary
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A highly promising technique to generate tissue constructs without using matrix is to culture cells in a simulated microgravity condition. Using a rotary culture system, we examined ovarian follicle growth and oocyte maturation in terms of follicle survival, morphology, growth, and oocyte function under the simulated microgravity condition.

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Keywords: Mouse Preantral Follicles In Vitro Growth Simulated Microgravity Follicle Isolation Follicle Encapsulation Alginate Beads Rotating Culture System In Vitro Culture Control Group Microgravity Treatment
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