Journal
/
Developmental Biology
/
Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro

DOI:
10.3791/57599-v

•

09:33 min

•

June 02, 2018

•

, , ,
1Program in Developmental Biology,Baylor College of Medicine, 2Center for Cell and Gene Therapy, Texas Children’s Hospital, and Houston Methodist Hospital,Baylor College of Medicine, 3Molecular and Cellular Biology Department,Baylor College of Medicine, 4Stem Cell and Regenerative Medicine Center,Baylor College of Medicine, 5McNair Medical Institute,Baylor College of Medicine

Chapters

  • 00:05Title
  • 00:42Preparation of Mouse Embryonic Day 10.5 Pancreatic Progenitors
  • 06:12Culturing Dissociated Progenitors to Form Pancreatoids
  • 06:50Results: Generation of Pancreatoids that Retain Native Mesenchyme and Develop Endocrine Cells
  • 08:37Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to generate insulin expressing 3D murine pancreatoids from free-floating e10.5 dissociated pancreatic progenitors and the associated mesenchyme.

Tags

PancreatoidsPancreatic ProgenitorsScaffold-freeThree-dimensionalInsulin-expressingMouseEndocrine CellsMesenchymeDissectionE10.5DispaseTrypsinIACUCUterusYolk SacPBS

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57599?article_type=v

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
check_url/57599?article_type=v

Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
check_url/57599?article_type=v

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
check_url/57599?article_type=v

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
check_url/57599?article_type=v

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies
check_url/57599?article_type=v

A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse
check_url/57599?article_type=v

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva
check_url/57599?article_type=v

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures
check_url/57599?article_type=v

Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells
check_url/57599?article_type=v

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Read Article