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Journal
/
Developmental Biology
/
无支架三维胰岛素表达 Pancreatoids 的
实验研究体外培养
的小鼠胰祖细胞
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors
In Vitro
Please note that all translations are automatically generated.
Click here for the English version.
无支架三维胰岛素表达 Pancreatoids 的
实验研究体外培养
的小鼠胰祖细胞
DOI:
10.3791/57599-v
•
09:33 min
•
June 02, 2018
•
Marissa A. Scavuzzo
,
Jessica Teaw
3,4
,
Diane Yang
3,4
,
Malgorzata Borowiak
2,3,4,5
1
Program in Developmental Biology
,
Baylor College of Medicine
,
2
Center for Cell and Gene Therapy, Texas Children’s Hospital, and Houston Methodist Hospital
,
Baylor College of Medicine
,
3
Molecular and Cellular Biology Department
,
Baylor College of Medicine
,
4
Stem Cell and Regenerative Medicine Center
,
Baylor College of Medicine
,
5
McNair Medical Institute
,
Baylor College of Medicine
Chapters
00:05
Title
00:42
Preparation of Mouse Embryonic Day 10.5 Pancreatic Progenitors
06:12
Culturing Dissociated Progenitors to Form Pancreatoids
06:50
Results: Generation of Pancreatoids that Retain Native Mesenchyme and Develop Endocrine Cells
08:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了一种生成胰岛素表达3D 小鼠 pancreatoids 从游离漂浮 e10.5 分离的胰祖细胞和相关间质的协议。
Tags
Pancreatoids
Pancreatic Progenitors
Scaffold-free
Three-dimensional
Insulin-expressing
Mouse
Endocrine Cells
Mesenchyme
Dissection
E10.5
Dispase
Trypsin
IACUC
Uterus
Yolk Sac
PBS
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