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Journal / Immunology and Infection / A Chronic Autoimmune Dry Eye Rat Model with…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
 

A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue

Article DOI: 10.3791/55592-v • 09:42 min • June 7th, 2017
June 7th, 2017 •
1,2, 3, 3, 1,2,4,5
1Singapore Eye Research Insitute, A Member of SingHealth, 2DUKE-National University of Singapore Medical School, 3Lee Kong Chian School of Medicine, Nanyang Technological University, 4Singapore National Eye Center, 5Yong Loo Lin School of Medicine, National University of Singapore

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Summary
Automatic Translation

This report describes a method to induce chronic experimental autoimmune dry eye in Lewis rats through immunization with an emulsion of rat lacrimal gland extract, ovalbumin, and complete Freund's adjuvant, followed by the injection of lacrimal gland extract and ovalbumin into the forniceal subconjunctiva and lacrimal glands six weeks later.

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Keywords: Autoimmune Dry Eye Rat Model Lacrimal Gland Effector Memory T Cells Ocular Disease Lymphocyte Trafficking Sprague Dawley Rat Lacrimal Gland Extract Freund's Adjuvant Ovalbumin
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