Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Application of Long-term cultured…
JoVE Journal
Immunology and Infection
Author Produced

This content is Open Access.

JoVE Journal Immunology and Infection
Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
 

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Article DOI: 10.3791/52833-v • 15:58 min • July 11th, 2015
July 11th, 2015 •
1,2, 1, 3, 3, 4, 1, 1
1Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 2Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, 3UK Veterinary Laboratories Agency, 4Visual Services, National Centers for Animal Health, Animal and Plant Health Inspection Service, United States Department of Agriculture

Chapters

Summary
Automatic Translation

Long-term cultured interferon-γ enzyme-linked immunospot assay is used as a measure of central memory responses and correlates with protective anti-mycobacterial vaccine responses. With this assay, peripheral blood mononuclear cells are stimulated with mycobacterial antigens and interleukin-2 for 14 days, enabling differentiation and expansion of central memory T cells.

Tags

Keywords: Interferon-γ ELISPOT Assay Effector T Cells Memory T Cells Central Memory T Cells Effector Memory T Cells Cattle Tuberculosis Vaccination Antigen Stimulation Long-term Culture
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter