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Journal
/
Immunology and Infection
/
长期培养的干扰素γ酶联免疫检测方法的评估效应和记忆T细胞应答的牛应用
JoVE Journal
Immunology and Infection
Author Produced
This content is Free Access.
JoVE Journal
Immunology and Infection
Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
Please note that all translations are automatically generated.
Click here for the English version.
长期培养的干扰素γ酶联免疫检测方法的评估效应和记忆T细胞应答的牛应用
DOI:
10.3791/52833-v
•
15:57 min
•
July 11, 2015
•
Mayara F. Maggioli
2
,
Mitchell V. Palmer
,
H. Martin Vordermeier
,
Adam O. Whelan
,
James M. Fosse
,
Brian J. Nonnecke
,
W. Ray Waters
1
Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service
,
United States Department of Agriculture
,
2
Department of Veterinary Pathology, College of Veterinary Medicine
,
Iowa State University
,
3
UK Veterinary Laboratories Agency
,
4
Visual Services, National Centers for Animal Health, Animal and Plant Health Inspection Service
,
United States Department of Agriculture
Chapters
00:00
Title
01:40
Methods
02:05
Long-term Culture
03:38
Elispot Assay Day 1
04:30
Elispot Assay Day 2
05:15
Adherent Cell Isolation
05:37
Flow Cytometry Plate Preparation
06:15
Harvest the 13 Day Long-term Cells
09:05
Elispot Assay Day 3
10:04
Alkaline Phosphatase Preparation
11:08
Substrate Solution Preparation
11:55
Reading the Plate
12:12
Performing Flow Cytometry
12:25
Results
14:37
Conclusions
Summary
Automatic Translation
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Automatic Translation
长期培养的干扰素γ酶联免疫斑点法被用作中央记忆应答的量度并且与保护性抗分枝杆菌疫苗的反应。用该测定中,外周血单核细胞刺激与分枝杆菌抗原和IL-2 14天,使分化和扩展中央记忆T细胞。
Tags
Interferon-γ
ELISPOT Assay
Effector T Cells
Memory T Cells
Central Memory T Cells
Effector Memory T Cells
Cattle
Tuberculosis
Vaccination
Antigen Stimulation
Long-term Culture
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