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Påføring av Long-term kultivert Interferon-γ Enzyme-linked immunospot analyse for Assessing Cell Responses Effector og Memory T i Cattle
This content is Free Access.
1Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service,United States Department of Agriculture, 2Department of Veterinary Pathology, College of Veterinary Medicine,Iowa State University, 3UK Veterinary Laboratories Agency, 4Visual Services, National Centers for Animal Health, Animal and Plant Health Inspection Service,United States Department of Agriculture
Chapters
- 00:00Title
- 01:40Methods
- 02:05Long-term Culture
- 03:38Elispot Assay Day 1
- 04:30Elispot Assay Day 2
- 05:15Adherent Cell Isolation
- 05:37Flow Cytometry Plate Preparation
- 06:15Harvest the 13 Day Long-term Cells
- 09:05Elispot Assay Day 3
- 10:04Alkaline Phosphatase Preparation
- 11:08Substrate Solution Preparation
- 11:55Reading the Plate
- 12:12Performing Flow Cytometry
- 12:25Results
- 14:37Conclusions
Long-term dyrket interferon-γ enzymbundet immunospot assay blir anvendt som et mål på sentralhukommelsesresponser og korrelerer med beskyttende antimykobakteriell vaksine responser. Med denne analysen, er perifere mononukleære blodceller stimulert med mykobakterielle antigener og interleukin-2 i 14 dager, som muliggjør differensiering og utvidelse av sentrale minne-T-celler.
