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蛋白质tRNA琼脂糖凝胶延迟测定分析<em>ñ</em<sup> 6</sup> - 磺酰氨基甲酰腺苷TcdA功能

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Biochemistry

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JoVE Journal Biochemistry

Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N⁶-threonylcarbamoyladenosine TcdA Function

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蛋白质tRNA琼脂糖凝胶延迟测定分析<em>ñ</em<sup> 6</sup> - 磺酰氨基甲酰腺苷TcdA功能

DOI:

10.3791/55638-v

•

08:03 min

•

June 21, 2017

•

Francisco J. Fernández^2,3, Sara Gómez, Sergio Navas-Yuste, Miguel López-Estepa, M. Cristina Vega

¹Department of Physical Chemical Biology, Center for Biological Research (CIB-CSIC),Spanish National Research Council (CSIC), ²Department of Immunology,Complutense University School of Medicine, ³Abvance Biotech srl

Chapters

00:05Title
01:13Preparation of the tRNA
02:44Lysis, Extraction, and Purification of tRNA(UUU)
04:05Sample Preparation
05:09Gel Electrophoresis and Staining to Observe tRNA-Protein Interaction
06:33Results: Evaluation of the Interaction Between TcdA and tRNA(UUU)
07:22Conclusion

Summary

Automatic Translation

介绍了通过琼脂糖凝胶阻滞测定技术生产tRNA（UUU）和tDNA（UUU）与酶TcdA复合物的分析方案。

Tags

Keywords: Protein-tRNA Interaction Agarose Gel Retardation Assay RNA-binding Proteins TcdA Function RNA Biology Protein-RNA Complexes E. Coli BL21 DE3 PET-23D EC Triple-U IPTG Induction Cell Lysis Acid Phenol Extraction

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