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Neuroscience
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使用高尔基Cox方法评估老年小鼠海马树突状复杂性
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method
Please note that all translations are automatically generated.
Click here for the English version.
使用高尔基Cox方法评估老年小鼠海马树突状复杂性
DOI:
10.3791/55696-v
•
09:44 min
•
June 22, 2017
•
Thomas R. Groves
2,3
,
Jing Wang
2
,
Marjan Boerma
2
,
Antiño R. Allen
2,3
1
Division of Radiation Health
,
University of Arkansas for Medical Sciences
,
2
Department of Pharmaceutical Sciences
,
University of Arkansas for Medical Sciences
,
3
Neurobiology & Developmental Sciences
,
University of Arkansas for Medical Sciences
Chapters
00:05
Title
00:55
Golgi Staining and Tissue Preparation
02:37
Tissue Sectioning
04:33
Post-staining Procedures
05:42
Mounting, Cleaning, and Covering
07:19
Results: Hippocampal Dendritic Complexity in Aged Mice
08:49
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里我们详细介绍一种高尔基体Cox协议。这种可靠的组织染色方法允许对海马中的细胞结构和整个大脑进行高质量的评估,同时进行最少的故障排除。
Tags
Golgi-Cox Method
Dendritic Complexity
Aged Mice
Neuroscience
Neuronal Structure
Golgi Staining
Mercuric Chloride
Post-impregnation Buffer
Vibratome
PBS
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