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使用高尔基Cox方法评估老年小鼠海马树突状复杂性

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method

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使用高尔基Cox方法评估老年小鼠海马树突状复杂性

DOI:

10.3791/55696-v

•

09:44 min

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June 22, 2017

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Thomas R. Groves^2,3, Jing Wang², Marjan Boerma², Antiño R. Allen^2,3

¹Division of Radiation Health,University of Arkansas for Medical Sciences, ²Department of Pharmaceutical Sciences,University of Arkansas for Medical Sciences, ³Neurobiology & Developmental Sciences,University of Arkansas for Medical Sciences

Chapters

00:05Title
00:55Golgi Staining and Tissue Preparation
02:37Tissue Sectioning
04:33Post-staining Procedures
05:42Mounting, Cleaning, and Covering
07:19Results: Hippocampal Dendritic Complexity in Aged Mice
08:49Conclusion

Summary

Automatic Translation

这里我们详细介绍一种高尔基体Cox协议。这种可靠的组织染色方法允许对海马中的细胞结构和整个大脑进行高质量的评估，同时进行最少的故障排除。

Tags

Golgi-Cox Method Dendritic Complexity Aged Mice Neuroscience Neuronal Structure Golgi Staining Mercuric Chloride Post-impregnation Buffer Vibratome PBS

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