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寡肽竞争测定磷酸化位点测定
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Biochemistry
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JoVE Journal Biochemistry
Oligopeptide Competition Assay for Phosphorylation Site Determination
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寡肽竞争测定磷酸化位点测定

DOI:
10.3791/55708-v

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09:16 min

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May 18, 2017

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, , , ,
1College of Pharmacy and Research Institute of Pharmaceutical Sciences,Seoul National University, 2College of Pharmacy and Institute of Pharmaceutical Science and Technology,Hanyang University

Chapters

  • 00:05Title
  • 01:05Site-directed Mutagenesis of pGEX-Nrf2
  • 03:45In Vitro Competitive 5′ Adenosine Monophosphate-activated Protein Kinase (AMPK) Assay
  • 06:13In Vitro AMPK Activity Assay with a Site-specific Mutant
  • 07:39Results: In Vitro AMPK Assays with Competitive Oligopeptides or a Site-specific Mutant Protein
  • 08:31Conclusion

Summary

Automatic Translation

Automatic Translation

肽竞争测定广泛用于各种分子和免疫学实验。本文描述了体外寡肽竞争激酶测定的详细方法和相关的验证程序，其可用于发现特定的磷酸化位点。

Tags

Oligopeptide Competition AssayPhosphorylation Site DeterminationCell SignalingPost-translational ModificationsSite-directed MutagenesisPCRDpnI DigestionBacterial TransformationDNA SequencingProtein Expression And Purification

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