JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Cancer Research
/
Ex 活体
人肺肿瘤切片中居民 CD8 T 淋巴细胞的共聚焦显微成像
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
Ex Vivo
Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
Ex 活体
人肺肿瘤切片中居民 CD8 T 淋巴细胞的共聚焦显微成像
DOI:
10.3791/55709-v
•
08:28 min
•
December 27, 2017
•
Elisa Peranzoni*
1
,
Houcine Bougherara*
1
,
Sarah Barrin*
1
,
Audrey Mansuet-Lupo
3
,
Marco Alifano
,
Diane Damotte
3
,
Emmanuel Donnadieu
1
Institut Cochin, Inserm U1016-CNRS UMR8104
,
Université Paris Descartes
,
2
Department of Pathology, Paris Centre University Hospitals
,
Université Paris Descartes
,
3
INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center
,
University Pierre and Marie Curie
,
4
Department of Thoracic Surgery, Paris Centre University Hospitals
,
University Paris Descartes
Chapters
00:05
Title
01:01
Agarose Embedding and Vibratome Slicing of Human Lung Tumors
03:51
Immunostaining of Resident CD8 T Cells and Tumor Cells
05:07
Imaging and Analyzing Resident CD8 T Cell Migration Within Human Lung Tumor Slices
07:03
Results: Distribution and Motility Patterns of Resident CD8 T Cells in Human Lung Tumor Tissues
07:53
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本协议描述了一种图像驻留肿瘤浸润 CD8 T 细胞标记的人肺肿瘤切片荧光耦合抗体的方法。这项技术允许 real-time 分析 CD8 T 细胞迁移使用共聚焦显微镜。
Tags
Ex Vivo Imaging
Resident CD8 T Lymphocytes
Human Lung Tumor Slices
Confocal Microscopy
Tumor Infiltrating CD8 T Cells
Tumor Immunology
T Cell Migration
Agarose
Vibratome
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/55709?article_type=v
体内
在肿瘤微环境中 pH 值、
p
O
2
、氧化还原状态、磷酸盐和谷胱甘肽浓度的 EPR 评价
check_url/55709?article_type=v
核素荧光报告基因成像在啮齿动物肿瘤模型中跟踪肿瘤进展
check_url/55709?article_type=v
肿瘤靶向免疫调节副病毒的设计与评价
check_url/55709?article_type=v
cd8
+
t 细胞诱导体外肿瘤细胞凋亡的实时检测--研究肿瘤浸润髓内细胞的免疫抑制作用
check_url/55709?article_type=v
利用人类诱导多能干细胞生成肿瘤抗原特异性T细胞
check_url/55709?article_type=v
超声成像对小鼠肺肿瘤进展的检测
check_url/55709?article_type=v
正交胰腺肿瘤的产生与肿瘤渗透T细胞细胞毒性的
外体
特征
check_url/55709?article_type=v
测量组织肿瘤组织切片的实时药物反应
check_url/55709?article_type=v
肿瘤细胞外侧尾静脉注射后肺转移的病理分析
check_url/55709?article_type=v
肿瘤移植用于评估小鼠肿瘤浸润CD8
+
T细胞的动力学
Read Article